Purpose: To evaluate the effect of fasting on anterior chamber depth, anterior chamber volume and corneal and lens density by Scheimpflug technology and to measure intraocular pressure changes. Methods: Totals of 50 healthy fasting individuals in Ramadan (study group) and 50 healthy non-fasting subjects (control group) were included. Central corneal thickness (CCT), anterior chamber depth (ACD), anterior chamber volume (ACV), corneal density (CD) and lens density (LD) were evaluated with Scheimpflug technology. Intraocular pressure (IOP) was measured with applanation tonometry. Both eyes of each participant were examined and also compared. Results: Comparison of groups: There was a statistically significant difference between CD, ACD, ACV and IOP (respectively, right/left eye: fasting: CD: 12.81 ± 0.76/12.73 ± 0.73; ACD: 2.92 ± 0.37 mm/2.93 ± 0.37 mm; ACV: 168.3 ± 41.82 mm3/183.34 ± 32.46 mm3; IOP: 13.80 ± 1.22 mmHg/14.88 ± 2.73 mmHg; non-fasting: CD: 13.28 ± 1.01/13.17 ± 0.77; ACD: 3.06 ± 0.31 mm/3.07 ± 0.31 mm; ACV: 167.46 ± 42.92 mm3/180.68 ± 31.45 mm3; IOP: 13.60 ± 1.65 mmHg/14.74 ± 2.93 mmHg) values on the right side (p < 0.01, p = 0.03, p = 0.04, p = 0.01, respectively). Although there was a statistically significant difference between the ACV, CD and IOP values on the left side (p < 0.01, p = 0.03, p = 0.01, respectively), no statistical significance was found for the ACV value on the left side (p = 0.08). Conclusion: The results demonstrated that, while fasting did not lead to any change in LD and CCT, it caused a small decrease in ACD and ACV, and a significant decrease in CD and IOP values. Scheimpflug technology seems to be a valuable tool for the evaluation of anterior segment changes in patients who are fasting.