Medicago sp. are often consumed as vegetable, salad and herbal tea as a form of fresh leaves and herbs. It is also very important in animal feeding, because it contains high percentage of protein and some important phytochemicals. In addition, traditionally, the leaves or other parts of the plant were used for treatment and preventing of kidney disorders, osteoporosis, anemia, diabetes, ulcer, coronary diseases, some cancers, and menopausal symptoms. In previous reports, it has been demonstrated that this plant has scavenging activity of the free radicals, which are involve in the development of the hypertension, Ischemia, neurodegenerative and rheumatoid diseases. In this study, the antioxidant, enzyme inhibitory activities of M. sativa L. collected in two growing period and phenolic substances that may be responsible for these activities were investigated. In this way, the beneficial effects of the plant will be revealed and a scientific work will be provided for the evaluation of the pharmaceutical and food industry.