Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Atıf Yapan Yayın
Exploring the chemical constituents and biological activities of leaf and twig extracts of two Amygdalus species from Turkey's flora: Cell-free, in vitro and molecular docking approaches
Scopus
Havuzumuzda 6 atıf almış
Wild species of the subgenus Amygdalus (genus Prunus and family Rosaceae) have been insufficiently studied with regard to their chemical components and their pharmacological potential. The present study aimed at the investigation of the chemical composition of leaves and twigs of A. orientalis Mill. and A. zielinskii Browicz, thus assessing the antioxidant and enzyme inhibiting potential, as well as to define the safety profile. Our results showed that extracts were rich in phenolics, and liquid chromatography-diode array detector-tandem mass spectrometry (LC-DAD-MS) analysis allowed the identification of phenolic acids, flavonoids, coumarins, proanthocyanins, amino acids, fatty acids and derivatives. The assessment of the antioxidant potential demonstrated that the ethyl acetate extract of A. zielinskii twigs recorded the highest anti-ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (1298.10 mg trolox equivalent (TE)/g), total antioxidant (4.98 mmol TE/g), Cu++(1211.47 mg TE/g) and Fe+++ (907.86 mg TE/g) reducing properties. In addition, it also had the highest anti-DPPH (2,2-diphenyl-1-picrylhydrazyl) activity (560.17 mg TE/g), together with its methanolic extract and that of A. orientalis twigs, whereas the best chelation power was exerted by the ethyl acetate extract of A. orientalis leaves (31.10 mg EDTAE/g). Regarding enzyme inhibition, methanolic extracts of the leaves and twigs of both species displayed the highest α-glucosidase and tyrosinase inhibitory activity, while ethyl acetate extracts of A. orientalis twigs and leaves revealed the best butyrylcholinesterase (2.35 mg galantamine equivalent (GALAE)/g) inhibitory activity. Docking studies demonstrated strong binding affinities of compounds like rutin, kaempferol derivatives, and isorhamnetin-3-O-rutinoside with the abovementioned enzymes, supporting the consistent inhibitory potential. Moreover, we investigated the potential cytotoxicity of these extracts in different human cell lines, thus proving their safety. These results highlight the potential of the two Amygdalus species as a new source of bioactive molecules for the development of health-promoting applications.
Atıf Yapan Makale Bilgileri
Kurumlar (6)
Harran Üniversitesi
Sanliurfa, Turkey
Khartoum University
Khartoum, Sudan
Selçuk Üniversitesi
Selçuklu, Turkey
Università degli Studi di Messina
Messina, Italy
Université de Lorraine
Nancy, France
University of Nyíregyháza
Nyíregyháza, Hungary