Analyzes of aflatoxin B1 and zearalenone in corn silage by high performance thin layer chromatography (HPTLC)-fluorodensitometric method

In this study development of experimented and verified method available for routine analyzes of a flatoxin B1 (AFB1) and zearalenone (ZON) contamination in corn silage by High Performance Thin Layer Chromatography (HPTLC)-Fluoro densitometric method; and determination of contamination in corn silage samples collected from all of the Central Anatolia provinces by this method were aimed. The validation of both methods used in AFB1 and ZON analyses was performed by the parameters of specificity, linearity, recovery, sensitivity and precision. In the my cotoxin analyses, the limits of sensitivity were determined 2 ppb for AFB1 and 12 ppb for ZON. The recovery was 74% AFB1 and 81% for ZON in corn silage. Chloroform + methanol + water development solution was used for AFB1 analyses; and HPTLC plates were used instead of TLC plates in ZON analyses. A total of 260 corn silage samples which were the crops of the year 2007 were collected in the dairy cattle and sheep enterprises in the provinces of Aksaray, Ankara, Çanki{dotless}ri{dotless}, Eskişehir, Karaman, Kayseri, Ki{dotless}ri{dotless}kkale, Ki{dotless}rşehir, Konya, Nevşehir, Niǧde, Sivas and Yozgat in Central Anatolia-Turkey between the dates of March 1 and May 31, 2008; and AFB1 and ZON levels in collected corn silage samples were analyzed. The tested and verified methods whose effectiveness was experimented on the silage samples were determined in laboratories for routine analyses. The result of toxicological analyzes, AFB1 and ZON contamination were not detected in any of the silage samples.