Determination of quality status of persimmon pollens stored at low temperatures

This study was carried out to determine the pollen quality status of 3 foreign pollinator and 2 native Persimmons (Diospyros kaki L.) genotypes at different storage temperatures for 1 year. Pollen was stored at +4 °C, 0 °C,-20 °C and-80 °C for 1 year, rate of viability and germination were determined every 4 months. 2, 3, 5 Triphenyltetrazolium Chloride (TTC) solution was used to determine the viability of the pollen, and agar in petri dish method was used to determine the germination rate of the pollen. The initial measurement of the genotypes ranged from 58.12% to 76.65% in pollen viability rates. At the end of the 12th month, the highest pollen viability at +4 °C, 0 °C,-20 °C, and-80 °C was determined to be 13.00 %, 42.01 %, 60.72 %, and 59.45 %, respectively. The pollen germination rates of the genotypes were found to be between 52.84 % and 67.49 % in the initial measurement. At the end of the 12th month, the highest germination rates were determined as 1% at +4 °C, 13.24 % at 0 °C, 23.87 % at-20 °C, and 24.99 % at-80 °C. At the end of the storage period, pollination potentials of genotypes were found to be inadequate at +4 °C and 0 °C, and relatively acceptable at pollination ability at-20 °C and-80 °C.