Serosurveillance of Neospora caninum and Brucella species in Dairy Cattle of Konya, Turkey

This study reports the seroprevalence of Neospora (N.) caninum and Brucella sp. in aborting and non-aborting dairy cattle in Konya province of Turkey. To this end, blood samples were collected from 560 cattle, 66 of which were not aborting and 494 were aborting, and sera were isolated from these samples through standard protocol. Antibodies against N. caninum were determined by using a commercial competitive ELISA (cELISA) kit. Brucella sp. antibodies were determined using the Rose Bengal Plate Test (RBPT). According to cELISA results, 222 of 560 cattle (39.64%) were seropositive for N. caninum antibodies. Of 494 aborting cattle samples, 213 (43.11%) were positive for N. caninum antibodies. Through RBPT, 89 of the 560 cattle tested were positive for Brucella sp. Of 494 aborting cattle, 79 (15.99%) were positive for Brucella sp. The seropositivity differences between N. caninum and Brucella sp. were statistically significant in aborting cattle (p < 0.001). The co-infection rate of N. caninum seropositivity with B. abortus was detected 9.5% in aborting cattle. In conclusion, seroprevalence of neosporosis and Brucella sp. was 39.64% and 15.89% through cELISA and RBPT, respectively in cattle of Konya.