Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide and Boric Acid on E.Faecalis Biofilm

Background: To evaluate the effects of hydrogen peroxide (H2O2) and boric-acid(BA) on E. faecalis biofilm with or without photo-activation by Germinator (Design for Vision, USA). Materials: Biofilm formation was provided on sterilized dentin discs. Five groups were created(n=8): 1.5%, 3% and 5% H2O2; 5 and 10% BA. Each was exposed to irrigation-solutions with or without photo-activation. ‘Biofilm killing-percentage’ (100%), (CFU/ml) was calculated, colony-counting was performed. One-way analysis of variance, Duncan, Tukey's tests were provided. Results: All irrigants reduced the number of viable-bacteria in biofilm (p<0.05). The results demonstrated that H2O2 was able to achieve 100% eradication of E. faecalis biofilm both with and without photoactivation. 10% BA with or without photo-activation was found more effective than 5% BA concentration (p<0.001). Effects of H2O2 with or without photo-activation on biofilm was higher than BA groups (p<0.001). Conclusions: H2O2 with or without photo-activation is an effective solution against E. faecalis biofilm. 5 and 10% BA seem promising against E. faecalis while 10% seems more effective regardless the effect of photo-activation.