3',4'-Dihydroxyflavonol has a positive effect on the cerebellum oxidant/antioxidant system and BDNF and transmitter levels in focal brain ischemia–reperfusion

Cerebral ischemia–reperfusion (I/R) occurs when blood flow is restored after a temporary interruption and may lead to brain dysfunction through oxidative stress and neurotransmitter imbalance. This study aimed to determine the effect of cerebral I/R and 1-week 3',4'-Dihydroxyflavonol (DiOHF) treatment on the antioxidant/antioxidant system and glutamate, GABA, and BDNF levels in cerebellum tissue in male rats. This study was performed on 28 Wistar-albino type male rats, which were formed as follows: 1-Control Group: No anesthesia or surgical procedure was applied to the animals in this group. 2-Sham Group: After general anesthesia was induced in the animals in this group, the carotid artery regions were opened and closed. After the application, solvent application was performed for 1 week (1 ml DiOHF vehicle). 3-Ischemia–Reperfusion Group: After general anesthesia, the carotid arteries were isolated in rats and ligated for 30 min, ischemia was induced, and then reperfusion was performed. 4-Ischemia–Reperfusion + DiOHF Group: After general anesthesia, the carotid arteries were ligated for 30 min and ischemia was performed in rats, then reperfusion was performed, and then DiOHF supplementation was performed for 1 week. After one week of treatment, the animals were sacrificed under general anaesthesia, and cerebellum tissues were taken. Malondialdehyde (MDA), glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), thioredoxin (TRx-2), glutamate, GABA, and brain-derived neurotrophic factor (BDNF) levels were evaluated in the relevant tissue. I/R increased MDA, glutamate, and GABA levels, while GPx, catalase, SOD, thioredoxin and BDNF levels were decreased. However, 1 week of 3',4'-Dihydroxyflavonol treatment corrected the changes that occurred with I/R. The study results show that 1 week of 3',4'-Dihydroxyflavonol treatment after I/R has a positive effect on cerebellum changes that occur in global brain I/R.