Effects on wound healing of different incision instruments: Experimental study

Objective. Wound healing effects of scalpel, electrocautery, peak plasmablade, which are used for skin incisions and intestinal incisions, were searched. Material and method. 36 wistar albino rats 10-week were used as experimental animals. 3 groups were created. There were 12 wistar albino rats in each groups. Laparotomy by scalpel and transection to ascending colon were applied in the group I. The segment, which was transected, was anastomosised at once. The same processes were applied by electrocautery in the group II. The same processes were applied by peak plasmablade in the group III. Experimental animals were sacrified at the 7th post-operative day. 1 ml blood was taken by cardiac puncture for MDA measurement and then relaparotomy was applied. Approximately 4 cm intestinal segment, which included anastomosis line, was resected. Then anastomotic bursting pressures were measured. 4x4 cm2 abdominal wall was removed to measure rupture breaking pressure of incision. Tissue samples were got for incision line's and anastomosis line's histopathological examinations. Results. In the group II, anastomotic bursting pressure and rupture breaking pressure of abdominal wall were found less than the groups I and III. The difference between the groups was statistically reasonable (p<0.05). MDA values of each groups weren't statistically different. At anastomosis and incision line, any reasonable difference was found in terms of 7 criterions, which were evaluated histopathologically. Conclusion. We think that scalpel and peak plasmablade can be used for incisions safely. Peak plasmablade supplies hemostasis. So peak plasmablade shortens operation duration.