The importance of asphodeline species on enzyme inhibition: Anti-elastase, anti-hyaluronidase and anti-collagenase potential

Asphodeline species are widespread in the inner Anatolia region in Turkey and used for the treatment of skin disorders, earaches and haemorrhoids. The aim of the present study is to investigate in vitro inhibitory effects of the extracts prepared from the stems, seeds, leaves and roots of Asphodeline brevicaulis subsp. brevicaulis var. brevicaulis, A. baytopae and A. cilicica on hyaluronidase, collagenase and elastase enzymes. Hyaluronidase, collagenase and elastase inhibitory effects of the extracts were performed by using in vitro enzyme inhibitory assays based on spectrophotometric evaluation. The methanol extract of the roots of A. cilicica displayed the highest hyaluronidase, collagenase and elastase inhibitory activities. On the other hand, the acetone extract of the roots of A. cilicica, the acetone extract of the leaves of A. brevicaulis subsp. brevicaulis var. brevicaulis, the acetone and the methanol extracts of seeds of A. baytopae, acetone and the methanol extract of the roots of A. baytopae, the aqueous extract of the stems of A. cilicica, the acetone and the methanol extracts of the leaves of A. cilicica, the aqueous extract of the roots of A. cilicica possessed significant collagenase, hyaluronidase and elastase inhibitory activities. In the present study, extracts of Asphodeline species significantly inhibited collagenase, elastase and hyaluronidase enzymes, suggesting their utilization for the treatment of wounds, cancer, cardiovascular diseases, inflammation, bone destruction and fibrosis, as well as skin aging.