Relationships between glucose-6-phosphate dehydrogenase, glutathione peroxidase, reduced nicotinamide adenine dinucleotide phosphate, total protein, malondialdehyde, total glutathione and vitamin C parameters in goat milkcells

In this study, to reveal the antioxidant potential of goat milk cells, the activities of glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx) and the levels of reduced nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), malondialdehyde (MDA), vitamin C (Vit C) and total protein (TP) in goat milk cells were determined and correlations between these parameters were evaluated. Milk samples were collected from 19 clinically healthy goats from a private goat farm. Briefly, milk cells were collected from milk by centrifugation and than they were sonicated. Supernatant G6PD, GPx activities and NADPH, tGSH, MDA, Vit C and total protein levels were determined by spectrophotometric methods. As regards correlations: milk cell MDA levels were positively correlated with milk cell tGSH (r=0, 725, p<0, 01), milk cell Vit C (r=0.622, P<0.01) and milk cell NADPH (r=0.763, P<0.01) levels. There was a positive correlation between milk cell GPx activity and milk cell NADPH levels (r=0.659, P<0.01). Milk cell tGSH levels were positively correlated with milk cell Vit C (r=0.615, P<0.05) and milk cell NADPH (r=0.846, P<0.01) levels. Milk cell NADPH levels was positively correlated with milk cell Vit C levels (r=0.791, P<0.01). As a conclusion, the antioxidant potential of goat milk cells were evaluated and discusses.