The Effects of Intracellular Vitamin C Concentrations on Bovine Neutrophils Functions In Vitro

Revue De Medecine Veterinaire · Aralık 1998

Özet

In this study, the effects of extracellular vitamin C on PMNL functions (phagocytic and microbicidal activity) and intracellular vitamin C levels were investigated. Neutrophils were obtained from eleven healthy Holstein heifers. Phagocytic and microbicidal activity of neutrophils were measured by fluorescent microscopic technique and intracellular vitamin C levels were determined with spectrophotometer. After first incubation (135 min., 37 °C) 149.76 %, 289.78 % and 462.76 % vitamin C increases were observed in PMNL, at 50, 150, 300 μM/L of vitamin C, added to incubation media respectively. All the results compared to the controls, phagocytosis and microbicidal activity of PMNL increased by different vitamin C concentrations. A definite enhancement in PMNL functions observed in this study may have attributed to vitamin C supplemantation.

2 atıf Aralık 1998

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