Effect of menbutone on oxidative stress in sheep

The objective of this research was to determine the effects of administering the choleretic-effective menbutone (genabilic acid) at the recommended dose and duration of serum oxidative status and biochemical parameter levels in sheep. As is known, changes in the oxidative status of living organisms can be observed in many drug applications, disease states, or metabolic disorders. In the study, menbutone was administered at a dose of 10 mg·kg–1 (slow IV, SID, 2 days) to 10 Central Anatolian Merino sheep. Blood samples were collected from the sheep before (0th hour) and at the 12th, 24th, and 48th hours after menbutone administration. Serums were obtained from the collected blood samples. Serum oxidative status parameters (8–hydroxy–2–deoxyguanosine, malondialdehyde, superoxide dismutase, glutathione peroxidase, catalase), troponin I, and creatine kinase-MB isoenzyme levels were measured using the ELISA method, while levels of serum routine biochemical parameters were measured using an autoanalyzer. Menbutone did not affect oxidative stress parameters (8–hydroxy–2–deoxyguanosine, malondialdehyde, superoxide dismutase, glutathione peroxidase, catalase) in sheep (P>0.05) but caused temporary increases (P<0.05) in serum triglyceride and cholesterol levels. However, no statistically significant changes were detected in other serum biochemical parameters (Troponin I, creatine kinase-MB isoenzyme, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, low-density lipoprotein, high-density lipoprotein, blood urea nitrogen, creatinine). In conclusion, it can be asserted that menbutone can be used safely in sheep without altering oxidative stress parameters, but monitoring serum triglycerides and cholesterol may be important in animals with metabolic disorders.