Objective: Somatostatin receptors (SSTRs) are G protein-coupled transmembrane receptors that serve as a specific molecular target for a number of radiopharmaceuticals utilized for the imaging of neuroendocrine tumors (NETs). 68Ga-DOTA-TATE is a somatostatin analog that demonstrates a high affinity for SSTR2. Pediatric malignancies, such as neuroblastoma, pheochromocytoma, and paraganglioma, have been shown to express SSTR2, and 68Ga-DOTA-TATE is currently being used to evaluate these pediatric neoplasms. We aimed to analyze the distribution pattern of 68Ga-DOTA-TATE based on age and location in pediatric patients. Methods: We retrospectively analyzed 247 consecutive 68Ga-DOTA-TATE whole-body PET/CT scans performed in our department from May 2015 to April 2024 in pediatric patients with known or suspected neuroblastoma, neuroendocrine malignancy, pheochromocytoma, and paraganglioma. 93 subjects were included in this study who were disease-free at the time of imaging and had no tracer-avid lesion on 68Ga-DOTA-TATE PET/CT. The patients were divided into four groups according to age: infant (0–2 years), pre-school (3–6 years), school (7–12 years), and adolescent (13–18 years). A comparison of the SUV values of each organ across age groups was performed. Results: The highest levels of physiological uptake were observed in the spleen across all age groups, except for infants, who demonstrated the highest SUV values in the kidneys. 68Ga-DOTA-TATE uptake in the parotid glands, submandibular glands, thyroid gland, thymus, liver, spleen, adrenal glands, stomach, intestines, uterus, prostate, and testes demonstrated a statistically significant increase in the adolescent age group. In contrast to all internal organs, the lowest SUV max values were observed for all growth plates within the adolescent age group. Conclusion: This study presents the bio-distribution pattern of 68Ga-DOTA-TATE in pediatric patients, according to age and location. The ranges of the SUVmax and SUVmean values of 68Ga-DOTA-TATE obtained in the various organs are of paramount importance for accurately diagnosing malignancy in 68Ga-DOTA-TATE PET/CT studies.