Social, cultural, and political ideologies have a significant impact on gender policies. In traditional societies, gender identities beyond those determined by an individual’s biological sex at birth are disregarded and stigmatized. In this respect, sport is of great significance for transgender individuals as a field of visibility and struggle. However, political decisions taken by international and national federations on sporting grounds lead to the exclusion of transgender athletes from sports activities. The present study examines the reactions of X users to the decision of World Athletics to ban transgender women from competing in the women’s category. In this direction, comments posted on the X accounts of traditional mass media organizations (n = 7) and digital news producers (n = 14) operating in Turkey who reported on World Athletics’ decision on X were analyzed through qualitative and quantitative content analysis. As a result of the study, it was determined that traditional mass media avoided reporting the event. Moreover, while most of the comments posted by users (93.35%) consisted of content in support of the decision, it was found that cultural (18.50%) and religious (17.44%) themes also stood out. It was also found that transgender athletes were stigmatized with discourses including keywords such as “mutant.”.