Online learning has been conducted in recent years, especially during crises. There have been some studies to increase its effectiveness. Acceptance of online learning is important and affects learners’ comfort and their future use. The purpose of this study was to adopt the acceptance of online learning scale into Turkish culture which was developed to determine students’ acceptance of online learning in a developing country Survey model was adapted in this study, and data were collected from 384 participants studying in a public university. Confirmatory Factor Analysis (CFA) was performed to check the validity of the scale and Cronbach’s alpha coefficient was calculated to assess the reliability of the adapted scale. Five-factor structure of the scale was confirmed, and multiple goodness-of-fit indices, X2/sd, CFI, RMSEA, SRMR, GFI, and TLI/NNFI were in the range of acceptance levels in the literature. The calculated Cronbach’s Alpha values ranged between.81 and.94 in the total and sub-factors, which were above the recommended value in the literature. The adapted scale could be used by researchers and educators to detect the acceptance of online learning at different intervals.