This research aims to explore the interaction between digital transformation and organizational culture in the Society 5.0 structuring in Turkey. The study's sample comprises business owners, senior managers from medium and large enterprises, academicians, and public employees, selected through purposeful sampling methods. Employing an explanatory-sequential design of mixed methods, data collection involved conducting surveys among these distinct sample groups. The quantitative analysis component of this study incorporates exploratory factor analysis, confirmatory factor analysis, correlation analysis, and Structural Equation Modeling (SEM). In the qualitative analysis section, the content analysis method was employed to pose semi-structured questions during interviews with 46 individuals. Code relationship analysis was applied to the data. All data were analyzed using SPSS 25, AMOS 24, and MAXQDA 20 software. According to the results of the quantitative study, digital transformation and organizational culture have a mutual influence. This influence is visible at all levels, with all dimensions and subdimensions influencing one another. On the other hand, the qualitative analysis demonstrated that Society 5.0 and its sub-dimensions influence both digital transformation and organizational culture, as well as their sub-dimensions. Furthermore, it was determined that understanding Society 5.0 necessitates digital transformation as well as a shift in organizational culture. Finally, organizations need to consider the developments within the scope of Society 5.0 and change their organizational structures accordingly.