The objective of this study was to develop a reliable and valid instrument to assess the professional competence of emergency medical technicians. The research sampling consists of 820 students who were in their last year of the First and Emergency Aid Program. The tools utilized for data collection included a Demographic Information Form, the Emergency Medical Technician Professional Competence Perception Scale (EMT-PCPS), and the Self-Efficacy-Competence Scale. To test the validity of the measuring tool’s structure, exploratory factor analysis (EFA), item analysis and confirmatory factor analysis (CFA) were used. Cronbach Alpha coefficients and test-retest results have been examined for reliability testing. According to the validity test results, the 4-factor structure of EMT-PCPS explains 70.45% of the total variance. According to the reliability analysis results, the item-total test correlation values were greater than.30, the Cronbach’s alpha coefficient was.97, and the correlation values between test-retest scores were medium to good (p<.01). The research found that the EMT-PCPS is a valid and reliable measuring instrument for analyzing the professional competence perception levels of Emergency Medical Technicians who are candidates to keep working in the profession.