Introduction: The primary aim of this study was to evaluate the effectiveness of piezocision and microosteoperforation (MOP) in accelerating the leveling of mandibular anterior teeth. The secondary aim was to evaluate the changes in periodontal parameters and the differences in patient comfort. Methods: Forty-five patients in the study were randomly divided into 3 groups. After bonding mandibular teeth, the patients in the first group (9 girls, 6 boys) underwent piezocision, and the patients in the second group (10 girls, 5 boys) underwent MOP. Patients in the third group (7 girls, 8 boys) comprised the control group. The patients were asked to complete a visual analog scale for pain assessment during the first week of treatment and to complete the Oral Health Impact Profile-14 questionnaire at the end of the week. Little's irregularity index was measured from plaster models taken at the start of the mandibular bonding process, 4 weeks, 8 weeks, 12 weeks, and 16 weeks after the mandibular bonding process. Periodontal parameters were also recorded at baseline and 16 weeks. Results: Our study results showed statistically significantly more orthodontic tooth movement in the piezocision group (mean difference, 7.60 ± 1.55) than in the control group (mean difference, 5.95±1.28) during the total study period (P = 0.015). In the MOP group, no significant difference in the alignment rate was observed at any time compared to the piezocision and control groups (P >0.05). Conclusions: The leveling of mandibular anterior teeth was accelerated by piezocision over 16 weeks, predominantly in the first 12 weeks, whereas MOP had no effect. It was concluded that piezocision is an acceptable procedure and has no destructive effect on the periodontal tissue.