Regenerative endodontic treatment has gained popularity in recent years thanks to successful treatment results. This treatment has goals such as eliminating symptoms in immature teeth, a continuation of the stopped root development, and vitality of teeth. It has been shown in many studies that root development, continues after regenerative endodontic treatment in immature teeth. Five different types of root response seen after this treatment were demonstrated by Chen et al. These are elongation and closure of the roots, blunt closure of the roots, open root apex, canal obliteration, and formation of a hard tissue barrier. However, unlike these root responses, a different type of root response, which is rarely seen, has been shown to continue its development by separating the root apex from the main root. In this study, the diagnosis, treatment, and follow-up of split root development, which is rare after regenerative endodontics, is explained.