Benzoxazoles are heterocyclic compounds containing fused benzene and oxazole rings, which have been reported to possess antibacterial, antitubercular, antifungal, antiviral, antioxidant and anticancer activities. In this study, the mutagenic properties of fifteen benzoxazole derivatives reported previously to exhibit broad antimicrobial and potent antitumor effects were investigated for their genotoxic potential by employing the Ames test. The Ames test was conducted using Salmonella typhimurium TA98, TA100 and TA102 tester strains in the standard plate incorporation assay with and without liver S9 fraction. The results were evaluated statistically and no mutagenic activity of test drugs was observed. Relationship between the structure and cytotoxic activity of compounds was evaluated using the SAR method based on data obtained via the Ames test system.