Aim: It was aimed to evaluate the burnout levels of the academicians and research assistants employed at Medical Faculty of Selcuk University and Necmettin Erbakan University, and to investigate the factors affecting the level of burnout. Methods: This study included total 894 subjects; 564 faculty members and research assistants working at Necmettin Erbakan University and 330 faculty members and research staff employed at Faculty of Medicine, Selçuk University. A questionnaire consisting of 50 questions containing sociodemographic characteristics of the participants, and the Maslach Burnout Inventory (MBI) were filled with face-to-face interviews. Results: Of the participants, 63.1% were working at the University of Necmettin Erbakan, and 36.9% at Selçuk University. While the mean emotional exhaustion (EE) (p<0.001) and depersonalization (DP) (p<0.001) scores of the physicians who work in basic medical sciences, the staff titled Prof. Dr. and Assoc. Prof. Dr., the physicians with administrative duties, the staff at/over 40 years old and the married physicians were statistically significantly lower than those of the staff employed in the other medical and surgical sciences, the staff with other titles, the physicians without administrative duties, the staff under 40 years old and the single staff respectively; personal success (PS) mean scores were statistically significantly higher (p<0.001). Conclusion: In particular, the supports reducing burnout and motivating should be provided and maintained to the physicians who are employed at such places especially as Faculties of Medicine which aims both to provide the highest level of health care in the region and to raise the physicians of the future.