The chromatic separation of adenine, adenosine, cytosine, phenol, benzene, and toluene were investigated by using 5,11,17,23-tert-butyl-25,27- bis(cyanomethoxy)-26-(chloroformyl)-28-hydroxy-calix[4]arene bonded aminopropyl silica (CDBAPS) as a stationary phase. The separation ability of the stationary phase was observed to be good for target species. The effect of non-polar calix[n]arene network was observed in chromatographic processes, and it was thought that, thanks to the relatively polar nitrile groups, further selectivity would be obtained in various chromatographic separations.