Background: The purpose of this study was to investigate irisin and myostatin responses to acute high-intensity interval exercise. Materials and methods: Ten male professional kick-boxers aged between 18 and 24 years and 10 sedentary males with similar age and body weight participated in the present study. Participants performed 4 × 30-s Wingate test separated with 4 min of rest. Blood samples were taken immediately before and after exercise, and 3 and 6 h of recovery. Results and conclusion: At rest, irisin levels were higher in the kick-boxers (p < 0.05). Immediately after the exercise, irisin levels were decreased in both groups (p < 0.05). A trend toward a return to baseline appeared after 3 h of recovery in the kick-boxers (p < 0.05). At rest, myostatin concentrations were not different between the groups (p > 0.05). Immediately after the exercise, myostatin levels were increased in both groups (p < 0.05). A trend toward a return to baseline appeared after 3 h of recovery in the kick-boxers (p < 0.05). Acute highintensity interval exercise decreased irisin levels and increased myostatin levels.