The objective of this investigation was to compare irisin concentration and its relation with oxidative stress markers, antioxidant enzymes and element levels in both male adolescent swimmers and sedentary controls. Twelve male swimmers aged between 11 and 18 years, who performed regular swimming training for at least 2 years and 12 age- A nd gender-matched sedentary controls participated in this study. After anthropometric measurements were performed, fasting venous blood samples were taken at rest. Irisin, malondialdehyde (MDA) as a marker of oxidative stress, superoxide dismutase (SOD) as a marker of antioxidant enzyme and elements (Zn, Cu, Se, Cr, Ni, Al, Fe) were analyzed in these samples. MDA levels were lower but Zn levels and Zn/Cu ratio were higher in the swimmers than in sedentary controls (p < 0.05). There was no statistically significant difference in the irisin levels and SOD activities between the groups (p > 0.05). The results of the current study suggested that exercise training has antioxidant effects and may reduce oxidative damage. Exercise training has also limited effects on irisin levels in the adolescents.