This study presents electrochemical determination of tyramine (TA) using a new electrochemical sensor (TiO2-Ag/PPy/GCE) based on TiO2-Ag/PPy nanocomposite modified glassy carbon electrode (GCE). In addition to SEM, AFM and XRD analysis, the presented sensor was studied with respect to its response to Fe(CN)6 4−/3− and characterized by CV and EIS techniques. Under the optimum conditions, TiO2-Ag/PPy/GCE showed a good linear relationship over a concentration range from 4.10 × 10-8 to 3.06 × 10-6 M with a detection limit of 2 × 10-8 M. The availability of the sensor was examined by analysis of TA in food samples.