In this study, the effect of barley malt process on antioxidant activity, carotenoid content, oil yield, phenolic compounds and fatty acid composition of barley, green malt and malt was investigated. The highest antioxidant activity (79.80%) and total phenolic content (122.43 mg/100 g) was observed in green malt. Carotenoid content of green malt (1.71 µg/g) was higher than those of barley and malt. Green malt had the maximum (+)-catechin (69.06 mg/100 g), 1,2-dihydroxybenzene (37.21 mg/100 g), quercetin (30.78 mg/100 g) and isorhamnetin (22.44 mg/100 g) content. Oil contents of samples ranged from 1.73 to 2.13% and showed increase with malting process. While barley lipids contained 18.53% palmitic, 19.94% oleic and 51.74% linoleic acids, malt oil contained 17.33% palmitic, 15.62% oleic and 56.56% linoleic acids. Linoleic acid content increased during malting process while oleic and palmitic acid content decreased.