We compared the C-banding pattern and the NOR distribution in 3 populations of N. xanthodon and N. ehrenbergi with 48 chromosomes from Turkey, with the aim to reveal variations between and within species. We have found distinct variation in the C-banding and the NOR distribution pattern among the 3 populations compared; each of them possessed its own specific chromosomal features. However, it is not possible to distinguish between intra- and interspecific variation, and the observed patterns cannot be utilized for unequivocal species recognition.