In this study, the distribution of Central Nervous System (CNS) tissue contamination on the joints of beef during cutting of 88 carcass halves in a private slaughterhouse was investigated. In conclusion, the first time in Turkey, the level of contamination of spinal cord tissue as CNS tissue on the joints of beef during cutting the carcasses into the parts was shown. Additionally, high level of contamination risk via the knives used for cutting carcasses, the cutting tables, the moving cutting tables and the aprons was detected.