Objective: Lymphadenopathy, is one of the most common presenting complaints in pediatricians routine. Parental concerns about the removal of the malignancy is a challenge for physicians. In our clinic, we aimed to evaluate retrospectively cases due to biopsy lymphadenopathy. Material and Methods: The medical records of the patients diagnosed with lymphadenopathy and underwent a biopsy between January 2011 and January 2014 in our clinic were examined retrospectively. The files of these patients were studied for the variables of age, gender, location, histopathology results, and symptoms. Results: 23 patients, 16 male and 7 female, had been conducted a diagnostic biopsy due to lymphadenopathy. The average age of the patients was 8.8 (1-17). Out of these 23 biopsies conducted, the histopathological findings in 10 patients suggested lymphadenitis, in 9 benignant lesions, and in 4 malignant lesions. Out of the 10 patients, diagnosed with lymphadenitis, 3 had chronic granulomatous lymphadenitis. Conclusion: Diagnosis can be sufficient most cases to be in detailed history and physical examination. Etiology in most cases is limited and a role in infections and benign structures such as nonspecific reactive hyperplasia. In order to clarify the etiology and and discard malignity in lymphadenopathies, a biopsy made according to the indication clears the path of the clinician.