The objective of this study was to find the relationship between bull sperm head dimensions, elongation, ellipticity and mitochondrial helix length in order to find a parameter for the measurement of sperm motion. Sperm cells were obtained from Holstein bulls and supravital stained with Nigrosin-Eosin then smeared on replicate slides. Sperm head length, head width, elongation, ellipticity and mitochondrial length were measured as 14.25±0.17, 7.27±0.23, 0.32±0.09, 1.98±0.18 and 20.30±0.15, respectively. But no statistically important correlations were found between these traits and mitochondrial lengths. © Medwell Journals, 2010.