Objectives: This study was done to evaluate mothers' level of knowledge regarding poisoning, to plan training for issues with an identified lack of knowledge, to collect required data regarding protection and approach issues on poisoning cases which may occur in children for various reasons. Methods: This descriptive study was performed after obtaining permission from the County Health Department and involved mothers who applied to Family Health Centers No. 1-7 between April 1st and May 31st 2012, and who agreed to participate in the study (n=290). The questionnaire was composed of three parts: "Personal Information Form," "House Poisoning Evaluation Form" and "Home Poisoning Prevention Knowledge Level Form." Results: Participant ages were between 16 and 50 years and the mean age was 33.09±7.10 years. The number of children ranged from 1 to 6, and 203 people had seven children under the age of six. 37.6% of the mothers were primary school graduates, while 74.5% were housewives. There was a significant relationship between the knowledge score of the mothers on poisoning and education, career, neighborhood, and social security (p < 0.05). Conclusions: Childhood poisoning is the most common cause of admission to the hospital. Protective precautions such as family education, storage of medication out of reach of children and use of secure lids are thought to be important.