Herein the removal and recovery of chromium anions from aqueous solutions by using nanofiltration pilot-scale equipment (Osmonics Sepa CF Membrane Cell) with a water-soluble amino calix[4]arene derivative was studied. To understand the selectivity, the authors also examined the retention of chromium anions in the presence of Cl-, NO3-, SO42-, HSO4-, CO32-, PO43-, H2PO4- anions in nanofiltration-complexation. From the results water-soluble amino calix[4]arene was effective and selective ligand for dichromate anions over nitrate anions, in a nanofiltration-complexation system at pH 2.5. Moreover, the recovery and reusability studies of dichromate and nitrate anions and also ligand were performed.