Background: Academic incivility is one of the most significant issues that disrupt the learning process for nursing students and adversely affects the quality of nursing care. Therefore, it is crucial to assess incivility within the nursing academic environment using valid and reliable measurement tools. The aim of this study is to translate and adapt the Incivility in Nursing Education-Revised (INE-R) Survey into Turkish and assess its validity and reliability for nursing students and faculty. Methods: The cross-cultural adaptation and validation of the INE-R were carried out within the framework of a methodological cross-sectional study. The Turkish validity and reliability of the INE-R survey, which assesses the behaviors of faculty incivility, were examined with 522 nursing students from 11 different universities, including students from the 2nd, 3rd, 4th year. Data were collected from April 20, 2021 to October 30, 2021. Results: Following the cross-cultural adaptation, the confirmatory factor analysis (CFA) confirmed that the Turkish version of the INE-R, with 24 items and a two-factor structure, showed a good fit. The reliability analysis revealed Cronbach’s alpha of 0.952 for the total score, 0.899 for the low-level incivility subscale, and 0.991 for the high-level incivility subscale. Item analysis indicated that all items had item-total correlation values above 0.30, with none removed. Additionally, removing any item did not improve the scale’s Cronbach’s alpha. The test-retest results showed no statistically significant differences. Conclusion: The Turkish adaptation of the INE-R demonstrates its validity and reliability as a valuable instrument for assessing the perceptions of Turkish nursing students regarding uncivil faculty behaviors, as well as the frequency of their exposure to such behaviors. Impact statement: INE-R survey has demonstrated psychometric reliability and validity, making it a valuable tool for assessing incivility within nursing academia.