In our research, acetamiprid, spinosad, and deltamethrin resistance levels were determined in Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae) populations collected from two different locations in potato cultivation areas of Niğde, Afyonkarahisar, and Konya provinces, where 34.8% of the amount was produced. The insecticides were applied using a spray tower at different concentrations. 3-4th instar larvae were subjected to residual effect tests. The susceptible population was obtained from Niğde Ömer Halisdemir University. Field populations were collected starting from June 2023. In the residual effect tests, at the end of the 24-hour exposure period, the LC50 values obtained from the susceptible population, Sandıklı, Şuhut, Konaklı, Çiftlik, Çumra, Selçuklu populations were determined as 1.507, 111.964, 89.617, 81.977, 73.563, 38.062, 7.409 mg ai l-1 for acetamiprid; 1.105, 21.035, 16.218, 11.982, 15.841, 8.183, 8.945 mg ai l-1 for deltamethrin and 4.738, 104.224, 96.404, 70.211, 64.147, 56.863, 37.639 mg ai l-1 for spinosad, respectively. The highest resistance rate was detected in the Sandıklı population as 74.30-fold, 19.04-fold, and 22-fold for acetamiprid, deltamethrin, and spinosad, respectively. Considering insecticide resistance, it is important to give priority to insecticides to which the pest has developed less resistance in chemical control of L. decemlineata.