This study explores the relationship between maternal employment guilt, job satisfaction, and perceived social support among working mothers. Data from 401 mothers with children aged 0–12 were collected via questionnaires and analyzed using SPSS and AMOS. Findings revealed that private sector mothers experienced higher guilt than those in the public sector. Mothers working fewer than 6 hours daily reported lower guilt and higher job satisfaction compared to those working 9 hours or more. Although no significant relationship was found between perceived social support and employment guilt, results indicated that job satisfaction decreased as guilt increased. The study highlights challenges in balancing work and family roles and underscores the need for family-friendly workplace policies. These policies could reduce maternal guilt and improve job satisfaction, offering practical insights for organizations to better support working mothers.