The aim of the study was to determine the effects of vaccination on the antibody titres of daily cattle seronegative and seropositive to Bovine Viral Diarrhea Virus (BVDV). For this purpose, 23 cattle were used in the study. Before the vaccination, 23 cattle from two different dams (consisted of 15 and 8 in each) were tested for presence of BVDV specific antibodies and antigens. In the study, 16 (69.6%) and 2 (8.7%) animals were found to seropositive and persistently infected [antigen (+) but antibody (-)], respectively. Remaining 5 cattle (21.7%) were detected as seronegative. Sixteen seropositive and five seronegative cattle were vaccinated with an inactive commercial BVDV vaccine. In the study, serum and blood samples were collected before and after vaccination and analysed for the precence of BVDV specific antibodies and antigens using Enzyme Linked Immunosorbent Assay (ELISA). Serum Neutralization Test (SNT) was also used to detect BVDV neutralizing specific antibodies. Antibody titres in serum samples collected from vaccinated seropositive animals were significantly (p = 0.001, p<0.001) increased compare to those of samples collected before vaccination detected by ELISA. Finally, it was thought that vaccination of seropositive cattle for BVDV may result in long-lasting and strong immunity compared to those of seronegative animals which may benefical to protect cattle against BVDV infection. © Medwell Journals, 2013.