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Chemical composition and biological activities of extracts from three Salvia species: S. blepharochlaena, S. euphratica var. leiocalycina, and S. verticillata subsp. amasiaca
Scopus
Toplam 118 atıf DOI
The genus Salvia has recently attracted great attention due to its notable biological activities. Within this context, in this study, the chemical characterization and biological effects of three extracts (dichloromethane (DCM), methanol (MeOH), and water) from three Salvia species (S. blepharochlaena (SB), S. euphratica var. leiocalycina (SE), and S. verticillata subsp. amasica (SV)) were assessed. For the chemical characterization, the qualitative and quantitative analysis of phenolic components in the methanol extracts was carried out by high-performance liquid chromatography with electrospray ionization mass spectrometry detection (HPLC-UV-ESI-MSn). Total phenolic, flavonoid and phenolic acid contents were also studied. Concerning the biological effects, antioxidant (DPPH and ABTS free radical scavenging; ferric (FRAP) and cupric (CUPRAC) reducing power; phosphomolybdenum, and metal chelating assays), enzyme inhibitory (cholinesterase, tyrosinase, amylase, glucosidase, lipase, and elastase) and cytotoxic effects (A-549 and MCF-7 cell lines) were evaluated. After the evaluation of the phytochemical profile by HPLC-UV-ESI-MSn, it was observed that the main compound in the analyzed extracts was rosmarinic acid, which was present at high concentrations, particularly in SV, which presented rosmarinic acid levels higher than the usual levels found in other Salvia species or related plants. Generally, the SV-water extract presented the strongest antioxidant abilities with higher levels of total bioactive compounds. However, the studied DCM extracts had higher enzyme inhibitory potentials compared with MeOH and water extracts. SE-DCM exerted the most potent cytotoxic effects, followed by SB-water and SB-MeOH extracts.
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Advantages of contemporary extraction techniques for the extraction of bioactive constituents from black elderberry (Sambucus nigra L.)flowers
Scopus
Havuzumuzda 58 atıf almış
Since ancient times, elderberry (Sambucus nigra L.)has been used in traditional medicine, but also as a supplement in various beverages and dishes. Although elderberry is a widespread species in the Balkan Peninsula, it is not sufficiently exploited and it is barely chemically and biologically characterized. The present study was focused on the phytochemical composition and biological effects of S. nigra flowers’ extracts, obtained by different techniques (microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE)and maceration (MAC))in the presence of different solvents (50% ethanol (EtOH)or water (H2O)). The phytochemical profiles of the obtained extracts were determined by LC–MS/MS and spectrophotometric techniques: rutin and 5-O-caffeoylquinic acid were found to be the major compounds in all extracts. To evaluate its biological potential, antioxidant, neuroprotective (acetyl – and butyrylcholinesterase inhibition (AChE and BChE)), antityrosinase and antilipase abilities were tested. MAE with 50% EtOH exhibited the strongest antioxidant activity, which was in good correlation with the high content of phenolics. The best AChE inhibitory effect was observed by MAC with H2O extract. However, the strongest tyrosinase inhibitors were 50% EtOH extracts. The presented results prove the high biological and industrial potential of S. nigra flowers and open new directions towards novel product design.
Atıf Yapan Makale Bilgileri
Kurumlar (3)
Selçuk Üniversitesi
Selçuklu, Turkey
University of Mauritius
Reduit, Mauritius
University of Novi Sad
Novi Sad, Serbia