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Direct electron transfer from glucose oxidase immobilized on polyphenanthroline-modified glassy carbon electrode
Scopus
Toplam 120 atıf DOI
This study reports direct electron transfer (DET) from immobilized glucose oxidase (GOx) via grafted and electropolymerized 1,10-phenanthroline monohydrate (PMH). The layer of poly-1,10-phenanthroline (PPMH) was gained via electrochemical deposition, which was used to create the PPMH-modified GC-electrode (PPMH/GC-electrode). Further, the GOx was immobilized on the PPMH/GC-electrode. The effect of surface-modification by the PPMH on the electron-transfer between enzyme and electrode-surface and some other electrochemical/analytical-parameters of newly designed enzymatic-electrode were evaluated. The PPMH/GC-electrode showed superior DET to/from flavine adenine dinucleotide cofactor of GOx, while some redox-compounds including ferrocene and K3[Fe(CN)6] were completely electrochemically inactive on the PPMH/GC-electrode. It was also found that the resulting GOx/PPMH/GC-electrode functioned as a " direct response type" glucose-biosensor. The biosensor showed excellent selectivity towards glucose and demonstrated good operational-stability. According to our best knowledge, this study is the first scientific report on electrochemical-polymerization of PMH on the GC-electrode in non-aqueous media followed by its application in the design of glucose-biosensor. © 2010 Elsevier B.V.
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Amperometric glucose biosensor based on glucose oxidase, 1,10-phenanthroline-5,6-dione and carbon nanotubes
Scopus
Havuzumuzda 13 atıf almış
A biosensor for glucose determination was fabricated by the immobilization of glucose oxidase (GOx) on carbon nanotubes (CNTs) and/or 1,10-phenanthroline-5,6-dione (PD) modified graphite rod electrodes (GOx/PD/CNTs/GR) and its amperometric response toward glucose was investigated under aerobic and anaerobic conditions. The sensitivity of the GOx/PD/CNTs/GR electrode was found to be higher compared to that of a PD-modified GR electrode without CNTs (GOx/PD/GR), implying that CNTs play an important role in the facilitation of electron transfer between the redox active site of GOx and the electrode surface. The GOx/PD/CNTs/GR biosensor exhibited a linear dependency on substrate concentration in a range from 0.0 until 50.0 mM of glucose with oxygen present and from 0.0 until 62.5 mM of glucose in the absence of oxygen. With oxygen present, the limit of detection (LOD) values were determined to be 5.4 and 8.0 mM, and the limit of quantitation values (LOQ) were calculated as 16.2 and 24 mM for GOx/PD/GR and GOx/PD/CNTs/GR, respectively. In the absence of oxygen, the LOD values were calculated as 4.2 and 10.7 mM, and the LOQ values were calculated as 12.6 and 32.1 mM for GOx/PD/GR and GOx/PD/CNTs/GR, respectively. When examining the interference effect of uric acid for GOx/PD/GR and GOx/PD/CNTs/GR electrodes, no significant changes in the amperometric response of the modified electrodes were observed up to 100.0 mM of uric acid.
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Kurumlar (4)
Fizinių ir Technologijos Mokslų Centras
Vilnius, Lithuania
Necmettin Erbakan Üniversitesi
Meram, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
Vilniaus Universitetas
Vilnius, Lithuania