Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen
Scopus
Toplam 121 atıf DOI
The aim of this study was to determine the effects of antioxidants such as reduced glutathione (GSH) and cysteine in Laiciphose® extender on semen parameters, fertilizing ability, lipid peroxidation (LPO) level and glutathione peroxidise (GPx) activity of post-thawed bull semen. Totally 54 ejaculates of three bulls were used in the study. Five groups, namely; GSH (0.5 and 2mM), cysteine (5 and 10mM) and control group, were conducted to test the antioxidants in Laiciphose®. Insemination doses were processed that each 0.25-mL straw contained 15×106 sperm. The addition of antioxidants did not present any significant effect on the percentages of post-thaw sperm morphology (acrosome and total abnormalities), subjective, CASA and progressive motilities, as well as sperm motility characteristics (VAP, VSL, VCL, LIN and ALH), compared to the control groups (P>0.05). GSH 0.5mM (55.5±7.38%) and cysteine 10mM (48±5.65%) led to lower rates of DNA damage, compared to control (P<0.05). As regards to MDA level, cysteine at 10mM dose gave the highest level (4.99±0.44nmol/L) (P<0.001). GPx activity was demonstrated to be higher level upon the addition of 5mM cysteine when compared to the other groups (P<0.05). With respect to fertility results based on 60-day non-returns, the supplementation of antioxidants did not present significant differences (P>0.05). The results of this study may provide an useful information for the future studies in this area. So, further studies could be suggested to achieve better information in terms of the DNA damage and fertilizing capacity of bull sperm frozen with effective antioxidants. © 2010.
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Atıf Yapan Yayın
Cryopreservation of bull sperm: Effects of extender supplemented with different cryoprotectants and antioxidants on sperm motility, antioxidant capacity and fertility results
Scopus
Havuzumuzda 66 atıf almış
The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations and trehalose (T) or cysteine (C; with/without) in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was divided into four equal aliquots and diluted using both of the Tris extenders with G (5% or 7%) or EG (3% or 5%). After that, each extenders were divided into three equal aliquots and diluted using both of the 5. mM C or 25. mM T, and control (without additives) was cooled to 4. °C and frozen in 0.25. ml French straws. The addition of 3% and 5% EG without antioxidants resulted in the least Computer-Assisted Sperm motility Analysis (CASA) motility as compared with the other groups. Treatment with 25. mM T in 3% EG beneficially effected acrosome morphology as compared with the other groups. Also, treatment with 3% EG with 25. mM T and 5% EG resulted in a greater rate of total abnormalities. Treatment with 3% G yielded a slightly greater percentage of membrane integrity by Hypo-Osmotic Swelling Test (HOST) assessment than that of the other groups. Treatment with 3% EG with 5. mM C resulted in the greatest concentration of malondialdehyde (MDA). The glutathione peroxidase (GPx) antioxidant activity was increased in the C-treatment groups when compared to the other groups. Treatment with 5% EG and 5. mM C resulted in less chromatin damage and detrimental impacts on tail moment. Treatment with 5% EG led to greater non-return rates of inseminated cows. However, this result was not considered to be statistically important.
Atıf Yapan Makale Bilgileri
Kurumlar (3)
Aksaray Üniversitesi
Aksaray, Turkey
Erciyes Üniversitesi
Kayseri, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey