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Screening of in vitro antioxidant and enzyme inhibitory activities of different extracts from two uninvestigated wild plants: Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata
Scopus
Toplam 130 atıf DOI
Introduction The importance of plants products in traditional medicine has been recognized for some time. Two plants of Turkish origin, Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata used as traditional Turkish medicine have remained uninvestigated for Alzheimer diseases and diabetes mellitus for their in vitro biological activity despite their use for sleep disorders. The antioxidant and enzyme inhibitory properties of these plants have not been reported. The aim of this study was to determine the total phenolics, flavonoids, and antioxidant as well as their enzyme inhibitory activity (in aqueous and solvent extracts). Methods Antioxidant assays used standard methods to assess phosphomolybdenum, free radical scavenging activity, reducing power, and metal chelating activity on ferrous ions. Additionally, the extracts were tested also for enzyme inhibitory activity (Cholinesterase, Tyrosinase, α-amylase, and α-glucosidase). Results Organic extracts showed the highest anti-cholinesterase (AChE, and BChE) activity, while aqueous extracts showed valuable Tyrosinase inhibitory activity. Total phenolics (expressed as gallic acid equivalents) in C. longiflorus subsp. longiflorus and C. minor subsp. auriculata were 46.2 and 25.4 mg in methanolic extracts, 27.5 and 26.2 mg in ethyl acetate extracts, and 37.9 and 46.6 mg in aqueous extracts, respectively. Similarly, total flavonoids (expressed as rutin equivalents) in C. longiflorus subsp. longiflorus and C. minor subsp. auriculata were 39.9 and 27.8 mg in methanolic extracts, 17.6 and 52.4 mg in ethyl acetate extracts, and 24.35 and 24.6 mg in aqueous extracts, respectively. Conclusion The reported results may be valuable for preparing new food supplements and can represent a good model for the development of new drug formulations.
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Enzyme inhibitory and antioxidant properties of six mushroom species from the Agaricaceae family
Scopus
Havuzumuzda Open Access 47 atıf almış
Mushrooms are excellent sources of nutraceuticals and have attracted much interest as functional foods. In this work the inhibitory capacity of six macrofungus (Coprinus comatus, Macrolepiota mastoidea, Agaricus campestris, Lycoperdon utriforme, Macrolepiota procera, and Leucoagaricus leucothites) against key enzymes related to diabetes type II (α-amylase, α-glucosidase), Alzheimer's disease [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] was assessed for the first time. The antioxidant properties (total antioxidant capacity, metal chelating, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assays) and phenolic contents of the selected mushroom species were also evaluated. We found that L. utriforme had the highest enzyme inhibitory activity (0.97 mg galantamine equivalents (GALAE)/g extract, 1.33 mg GALAE/g extract, 0.22 mmol acarbose equivalents (ACAE)/g extract, and 2.97 mmol ACAE/g extract against AChE, BChE, α-amylase, and α-glucosidase, respectively). A widely consumed mushroom species namely A. campestris possessed the highest phenolic content (15.63 mg GAE/g extract) and the maximum antioxidant potential against DPPH, ABTS, FRAP, and CUPRAC. Experimental data collected in the present study support the use of these mushrooms specially L. utriforme and A. campestris as functional foods for the management and/or prevention of diabetes type II, Alzheimer's disease, and oxidative stress related complications.
Atıf Yapan Makale Bilgileri
Kurumlar (3)
Ankara Üniversitesi
Ankara, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
University of Mauritius
Reduit, Mauritius