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Screening of in vitro antioxidant and enzyme inhibitory activities of different extracts from two uninvestigated wild plants: Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata
Scopus
Toplam 129 atıf DOI
Introduction The importance of plants products in traditional medicine has been recognized for some time. Two plants of Turkish origin, Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata used as traditional Turkish medicine have remained uninvestigated for Alzheimer diseases and diabetes mellitus for their in vitro biological activity despite their use for sleep disorders. The antioxidant and enzyme inhibitory properties of these plants have not been reported. The aim of this study was to determine the total phenolics, flavonoids, and antioxidant as well as their enzyme inhibitory activity (in aqueous and solvent extracts). Methods Antioxidant assays used standard methods to assess phosphomolybdenum, free radical scavenging activity, reducing power, and metal chelating activity on ferrous ions. Additionally, the extracts were tested also for enzyme inhibitory activity (Cholinesterase, Tyrosinase, α-amylase, and α-glucosidase). Results Organic extracts showed the highest anti-cholinesterase (AChE, and BChE) activity, while aqueous extracts showed valuable Tyrosinase inhibitory activity. Total phenolics (expressed as gallic acid equivalents) in C. longiflorus subsp. longiflorus and C. minor subsp. auriculata were 46.2 and 25.4 mg in methanolic extracts, 27.5 and 26.2 mg in ethyl acetate extracts, and 37.9 and 46.6 mg in aqueous extracts, respectively. Similarly, total flavonoids (expressed as rutin equivalents) in C. longiflorus subsp. longiflorus and C. minor subsp. auriculata were 39.9 and 27.8 mg in methanolic extracts, 17.6 and 52.4 mg in ethyl acetate extracts, and 24.35 and 24.6 mg in aqueous extracts, respectively. Conclusion The reported results may be valuable for preparing new food supplements and can represent a good model for the development of new drug formulations.
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Viola Odorata Extracts: Phytochemical Profiling, Antioxidant Capacity, Enzyme Inhibition, and COX-2/NOS-2-Mediated Anti-Inflammatory Effects
Scopus
Havuzumuzda
In the present study, the phytochemical and bio-pharmacological effects of the species Viola odorata were explored. Different polarity extracts, namely water and methanol were assayed for their content in phenolic compounds, enzyme inhibition and scavenging/reducing properties. Additionally, through a validated ex vivo model of colon inflammation, the effects of water and methanol extracts were assayed on Escherichia coli lipopolysaccharide (LPS)-induced up-regulation of both cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (NOS-2) gene expression. The results of the study indicate that methanol extract was the richest in terms of total phenols (37.26 mg GAE/g) and flavonoids (56.90 mg RE/g). Indeed, the methanol extract was the most effective as antiradical (DPPH: 24.91 mg TE/g and ABTS: 66.62 mg TE/g) but also as enzyme inhibitor toward cholinesterases (AChE: 3.23 mg GALAE/g and BChE: 5.02 mg GALAE/g), tyrosinase (133.29 mg KAE/g), glucosidase (0.27 mmol ACAE/g), and amylase (0.82 mmol ACAE/g). Transcription factors regulating NOS2 and COX-2 were used to construct a regulatory network, and five major phytochemicals were screened for target prediction. Selected proteins were subjected to molecular docking, revealing strong interactions between kaempferol-3-O-glucoside and rutin with BChE, NF-κB, and STAT3. The V. odorata extracts were also effective in blunting the LPS-induced gene expression of both COX-2 and NOS-2; thus, confirming enzyme inhibition effects, anti-inflammatory and antioxidant properties. The results suggest that this species is a valuable source of bioactive compounds for developing health-promoting applications.
Atıf Yapan Makale Bilgileri
Kurumlar (5)
Harran Üniversitesi
Sanliurfa, Turkey
Marmara Üniversitesi
Istanbul, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
Università degli Studi di Camerino
Camerino, Italy
University of G. d'Annunzio Chieti and Pescara
Chieti, Italy