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Screening of in vitro antioxidant and enzyme inhibitory activities of different extracts from two uninvestigated wild plants: Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata
Scopus
Toplam 130 atıf DOI
Introduction The importance of plants products in traditional medicine has been recognized for some time. Two plants of Turkish origin, Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata used as traditional Turkish medicine have remained uninvestigated for Alzheimer diseases and diabetes mellitus for their in vitro biological activity despite their use for sleep disorders. The antioxidant and enzyme inhibitory properties of these plants have not been reported. The aim of this study was to determine the total phenolics, flavonoids, and antioxidant as well as their enzyme inhibitory activity (in aqueous and solvent extracts). Methods Antioxidant assays used standard methods to assess phosphomolybdenum, free radical scavenging activity, reducing power, and metal chelating activity on ferrous ions. Additionally, the extracts were tested also for enzyme inhibitory activity (Cholinesterase, Tyrosinase, α-amylase, and α-glucosidase). Results Organic extracts showed the highest anti-cholinesterase (AChE, and BChE) activity, while aqueous extracts showed valuable Tyrosinase inhibitory activity. Total phenolics (expressed as gallic acid equivalents) in C. longiflorus subsp. longiflorus and C. minor subsp. auriculata were 46.2 and 25.4 mg in methanolic extracts, 27.5 and 26.2 mg in ethyl acetate extracts, and 37.9 and 46.6 mg in aqueous extracts, respectively. Similarly, total flavonoids (expressed as rutin equivalents) in C. longiflorus subsp. longiflorus and C. minor subsp. auriculata were 39.9 and 27.8 mg in methanolic extracts, 17.6 and 52.4 mg in ethyl acetate extracts, and 24.35 and 24.6 mg in aqueous extracts, respectively. Conclusion The reported results may be valuable for preparing new food supplements and can represent a good model for the development of new drug formulations.
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Experimental and theoretical exploration of biological activities of different extracts of Equisetum arvense: Enzyme inhibition, DFT, molecular docking and molecular dynamics analyses
Scopus
Havuzumuzda 1 atıf almış
In this study, the antioxidant and enzyme inhibition capacities of acetone, ethanol, and water extracts of E. arvense were comprehensively investigated using both experimental and theoretical approaches. The phytochemical constituents of the extracts were characterized by a validated LC-MS/MS method, which identified 53 bioactive molecules commonly found in medicinal plants, including prominent compounds such as Isoquercitrin, Quinic acid, Acacetin, Aconitic acid, Caffeic acid, and Protocatechuic acid. Among the tested extracts, acetone exhibited the highest levels of phenolic and flavonoid contents, and accordingly demonstrated the strongest antioxidant activity as evaluated by DPPH, ABTS, CUPRAC, FRAP, and other assays. Enzyme inhibition studies showed the strongest effect for the ethanol extract in cholinesterase inhibition tests. Similarly, the greatest inhibition of tyrosinase was observed with the ethanol extract. To elucidate the molecular basis of these bioactivities, dominant compounds were subjected to density functional theory (DFT) calculations and molecular docking simulations against key enzymes. Theoretical results confirmed that Isoquercitrin and Acacetin possess favorable electronic and binding properties, suggesting their role in the observed bioactivities. These findings highlight E. arvense as a promising natural source of multifunctional phytochemicals with potential applications in managing oxidative stress and metabolic disorders.
Atıf Yapan Makale Bilgileri
Kurumlar (4)
Cumhuriyet Üniversitesi
Sivas, Turkey
Dicle Üniversitesi
Diyarbakir, Turkey
Ege Üniversitesi
Izmir, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey