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Screening of in vitro antioxidant and enzyme inhibitory activities of different extracts from two uninvestigated wild plants: Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata
Scopus
Toplam 130 atıf DOI
Introduction The importance of plants products in traditional medicine has been recognized for some time. Two plants of Turkish origin, Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata used as traditional Turkish medicine have remained uninvestigated for Alzheimer diseases and diabetes mellitus for their in vitro biological activity despite their use for sleep disorders. The antioxidant and enzyme inhibitory properties of these plants have not been reported. The aim of this study was to determine the total phenolics, flavonoids, and antioxidant as well as their enzyme inhibitory activity (in aqueous and solvent extracts). Methods Antioxidant assays used standard methods to assess phosphomolybdenum, free radical scavenging activity, reducing power, and metal chelating activity on ferrous ions. Additionally, the extracts were tested also for enzyme inhibitory activity (Cholinesterase, Tyrosinase, α-amylase, and α-glucosidase). Results Organic extracts showed the highest anti-cholinesterase (AChE, and BChE) activity, while aqueous extracts showed valuable Tyrosinase inhibitory activity. Total phenolics (expressed as gallic acid equivalents) in C. longiflorus subsp. longiflorus and C. minor subsp. auriculata were 46.2 and 25.4 mg in methanolic extracts, 27.5 and 26.2 mg in ethyl acetate extracts, and 37.9 and 46.6 mg in aqueous extracts, respectively. Similarly, total flavonoids (expressed as rutin equivalents) in C. longiflorus subsp. longiflorus and C. minor subsp. auriculata were 39.9 and 27.8 mg in methanolic extracts, 17.6 and 52.4 mg in ethyl acetate extracts, and 24.35 and 24.6 mg in aqueous extracts, respectively. Conclusion The reported results may be valuable for preparing new food supplements and can represent a good model for the development of new drug formulations.
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Enzyme inhibition andantioxidant properties of different Melissa officinalis L. extracts: experimental, DFT, molecular docking and molecular dynamics based analyses
Scopus
Havuzumuzda
The present paper focuses on the analyses of enzyme Inhibition and antioxidant properties of acetone, ethanol and water extracts of Melissa officinalis L. The biologically active molecular components of the mentioned extracts of the plant were reported with the help of through validated LC-MS/MS method. Validated LC–ESI–MS/MS analysis identified quinic acid, protocatechuic acid, protocatechuic aldehyde, vanillin, p-coumaric acid, naringenin, luteolin, apigenin, kaempferol, hesperetin, and acacetin as representative phytochemicals exhibiting distinct solvent-dependent distribution patterns. To test the antioxidant capacity of the extracts, several popular antioxidant capacity determination methods were used, including DPPH, ABTS, CUPRAC, and FRAP assays. The acetone extract exhibited the strongest antioxidant activity, showing DPPH, ABTS, CUPRAC, and FRAP values of 195.10, 246.39, 467.09, and 266.96 mg TE/g, respectively. Enzyme inhibition studies demonstrated that the ethanol extract showed the highest inhibitory activity against AChE (2.88 mg GALAE/g), BChE (1.92 mg GALAE/g), tyrosinase (55.90 mg KAE/g), α-amylase (0.18 mmol ACAE/g), and α-glucosidase (3.35 mmol ACAE/g). In the theoretical part of the study, chemical reactivity analyses about the major molecular components in the extracts were done using Density Functional Theory (DFT) based parameters and electronic structure principles. In addition, the strength and mechanisms of interaction of these molecules with the enzymes studied in the experimental part were tested through molecular docking and molecular dynamics simulations. Experimental and computational analyses were supported each other.
Atıf Yapan Makale Bilgileri
Kurumlar (4)
Cumhuriyet Üniversitesi
Sivas, Turkey
Dicle Üniversitesi
Diyarbakir, Turkey
Ege Üniversitesi
Izmir, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey