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Sideritis galatica Bornm.: A source of multifunctional agents for the management of oxidative damage, Alzheimer's's and diabetes mellitus
Scopus
Toplam 130 atıf DOI
The antioxidant and enzyme inhibitory potential of different solvent extracts (petroleum ether, ethyl acetate, methanol and water) from Sideritis galatica were evaluated. Cholinesterase, α-amylase and α-glucosidase inhibitory activities of the extracts were tested by microtiter plate assays. Antioxidant abilities were tested using free radical scavenging (DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid) and NO), reducing power (FRAP (ferric reducing antioxidant power) and CUPRAC (cupric reducing antioxidant capacity)), total antioxidant capacity and chelating assays. Methanol and water extracts showed higher phenolic content, DPPH and ABTS scavenging activity and reducing power activities, while the petroleum ether and ethyl acetate extract had the highest inhibition abilities on the enzymes. 18 phenolic components in these extracts were detected by using high performance liquid chromatography-diode array detector (HPLC-DAD). Results obtained in this work indicate that S. galatica may be useful as a source of natural agents for the management of oxidative process, Alzheimer's disease and type II diabetes.
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Bioactivities of Achillea phrygia and Bupleurum croceum based on the composition of phenolic compounds: In vitro and in silico approaches
Scopus
Havuzumuzda 29 atıf almış
This study presents the effects of the Achillea phrygia Boiss. et Bal. and Bupleurum croceum Fenzl. extracts obtained by different solvents (ethyl acetate, methanol and water) on selected enzyme inhibitory effects and antioxidant ability with screening bioactive compounds. Total and individual bioactive compounds were detected by spectrophotometric and HPLC-DAD techniques. Antioxidant abilities were evaluated by different methods including free radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating. Enzyme inhibitory effects were tested against cholinesterases, tyrosinase, amylase, glucosidase and lipase. Total phenolic contents were ranged from 20.52 mgGAE/g extract (B. croceum methanol extract) to 41.13 mgGAE/g extract (A. phrygia methanol extract). Generally, methanol and water extracts showed the strongest antioxidant abilities, while the ethyl acetate extracts had the most promising enzyme inhibitory effects. HPLC analysis revealed the abundance of some phenolics including rutin, quercetin, sinapic acid and chlorogenic acid, respectively. These components were also assessed using molecular modelling with the aim to study their docking properties on a set of six enzymes used in this study. Overall, these species could be suggested as valuable sources of natural-bioactive agents for developing new functional, pharmacological and health-promoting ingredients.
Atıf Yapan Makale Bilgileri
Kurumlar (4)
Ege Üniversitesi
Izmir, Turkey
Marmara Üniversitesi
Istanbul, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti, Italy