Kurumun Atıf Alan Makalesi
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Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen
Scopus
Toplam 132 atıf DOI
Oxidative damage to sperm resulting from reactive oxygen species generated by the cellular components of semen during liquid storage is possibly one of the main causes for the decline in motility and fertility during storage-the other detrimental cause is low temperature on the destabilisation of sperm membrane structure. The aim of this study was to determine the effects of the addition of the anti-oxidants taurine and glutathione (GSH), and the membrane structure stabiliser, trehalose, on sperm viability during low temperature liquid storage. A total number of 36 ejaculates were collected using the artificial vagina from four Chios rams and nine replicates of the ejaculates were diluted with a Tris-based extender containing additives as the control. The sperm motility, percentage abnormal sperm, plasma membrane intact sperm and the hypo-osmotic swelling test (HOST) were determined during storage of semen at 5 °C for a period of 0, 6, 24 and 30 h of liquid storage, respectively. Trehalose at a level of 50 mM provided the best maintenance of motility at 6 and 30 h (P < 0.05), and gave the highest percentage (69.0 ± 2.0% and 64.6 ± 1.8%, respectively) of viable sperm at 24 and 30 h (P < 0.01). Trehalose treatment at a concentration of 50 mM also resulted in the highest percentage of membrane-intact sperm (53.7 ± 2.9%) after performing HOST at 30 h. The anti-oxidant treatments GSH 5-10 mM and taurine at 50 mM provided a significant improvement in sperm survival during the 6 h of liquid storage at 5 °C (P < 0.05). In conclusion, many aspects of sperm protection, e.g. sperm motility, viability and membrane stabilisation of the sperm cells during relative low temperature storage, are the key factors determining the preservation of sperm function. Future efforts toward improving function of ram sperm kept in low temperature storage should concentrate on anti-oxidant additives. The results of this study provide a new approach to the preservation of sperm from rams of the Chios and related breeds, and so contribute to the improvement of these breeds for the world sheep industry. © 2007 Elsevier B.V. All rights reserved.
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Atıf Yapan Yayın
Influence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5 °C
Scopus
Havuzumuzda 38 atıf almış
Ejaculates were collected from six Merino rams with the aid of an artificial vagina twice a week. The ejaculates containing spermatozoa with >80% forward progressive motility and concentrations higher than 2 × 109 spermatozoa/ml were pooled. The present study included two experiments. In experiment 1, each pooled ejaculate was divided into four equal aliquots and diluted (37 °C) with the Tris based extender, containing 0 (control), 0.5, 1 and 2 mM lycopene, at a final concentration of approximately 400 × 106 sperms/ml (single step dilution), In experiment 2, cysteamine at concentrations of 0 (control), 0.5, 1 and 2 mM, was used as an additive in the extender, and the procedure explained above was applied for the division of aliquots and the dilution of semen. Diluted semen samples were kept in glass tubes and cooled from 37° to 5 °C in a cold cabinet, and maintained at 5 °C. Sperm and oxidative stress parameters were evaluated after 0, 24, 48 and 72 h of storage at 5 °C. The extender supplemented with 0.5 mM lycopene resulted in higher mitochondrial activity rate (p < 0.05) in comparison to the control group at 72 h of storage. Lycopene at 0.5 mM dose led to higher sperm motility rate (p < 0.05) when compared to 2 mM lycopene group at 72 h of liquid storage. As regards oxidative stress parameters, only 2 mM lycopene increased total glutathione levels (p < 0.05) at 0 h of storage. The extender supplemented with 1 mM cysteamine gave higher motility (p < 0.05) at 48 h compared to control. As regards oxidative stress parameters, 1 and 2 mM cysteamine at 48 h and 1 mM cysteamine at 72 h increased total glutathione levels (p < 0.05) compared to control groups. Cysteamine at 1 and 2 mM doses decreased lipid peroxidation (p < 0.05) at 0 h of liquid storage compared to control. Our data suggest that lycopene at 0.5 and 2 mM and cysteamine at 1 and 2 mM doses can be added to Tris based extender for improving the ram sperm motility, viability, mitochondrial activity and oxidative stress parameters during the liquid storage.
Atıf Yapan Makale Bilgileri
Kurumlar (9)
Afyon Kocatepe Üniversitesi
Afyonkarahisar, Turkey
Bahri Dagdas International Agricultural Research Institute
Konya, Turkey
Burdur Mehmet Akif Ersoy Üniversitesi
Burdur, Turkey
Erciyes Üniversitesi
Kayseri, Turkey
Mustafa Kemal Üniversitesi
Antakya, Turkey
Pamukkale Üniversitesi
Denizli, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
T.C. Tarim ve Köyişleri Bakanliği
Antakya, Turkey
Turkish Republic Prime Ministry Directorate General of Foundations
Ankara, Turkey