Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
Crocus sativus L. stigmas and byproducts: Qualitative fingerprint, antioxidant potentials and enzyme inhibitory activities
Scopus
Toplam 133 atıf DOI
Saffron (Crocus sativus L.) has been previously reported to be active as a protective agent in multiple experimental models of oxidative stress, inflammation and cancer. These findings refer to the protective effects of stigmas, not byproducts such as tepals and anthers. In this context, the aims of the present work were to characterize the phytochemical profile of saffron stigmas (CST) and high quality byproducts (tepals + anthers - CTA) extracts. Additionally, we studied the antioxidant and chelating effects of CST and CTA extracts by preliminary in vitro assay. The antioxidant activity was further investigated through the evaluation of reactive oxygen species (ROS) levels and lactate dehydrogenase (LDH) activity on mouse myoblast (C2C12) and human colon cancer (HCT116) cell lines. Additionally, we evaluated CST and CTA extract treatment on cholinesterases, α-glucosidase and α-amylase activity, in vitro. Finally, we studied the effects of CST extract on malondialdehyde (MDA) level in rat colon specimens challenged with E. coli lipopolysaccharide (LPS). We observed that water CST extracts are rich in phenolic content, whereas for CTA the olive oil was the elective extraction solvent. As expected, water CST extracts were the most effective in reducing hydrogen peroxide-induced oxidative stress in both cell lines and in vitro assays. Furthermore, both CST and CTA water extracts reduced the LDH activity in HCT116 cells challenged with hydrogen peroxide and LPS-induced MDA levels in rat colon specimens. Concluding, the present findings showed protective effects exerted by CST and CTA extracts in in vitro and ex vivo models of inflammation and oxidative stress.
Atıf Kaynağı
Atıf Yapan Yayın
Polyphenolic composition, enzyme inhibitory effects ex-vivo and in-vivo studies on two Brassicaceae of north-central Italy
Scopus
Havuzumuzda 67 atıf almış
In this study, three different extracts (soxhlet, microwave and decoction) from two species of broccoli: Brassica oleracea L. convar. Italica botrytis (L.) Alef. var. cymosa Duch. (Broccolo Fiolaro) and Brassica oleracea acephala L. convar. acephala (DC.) Alef. var. sabellica L. (Cavolo Nero), which are commonly spread in north-central Italy, were tested for their enzyme inhibitory effects. Enzyme inhibitory effects were investigated against cholinesterases, tyrosinase, α-amylase and α-glucosidase. The soxhlet extracts had the highest inhibitory AChE effects with 1.08 mgGALAE/g (in Cavolo Nero) and 0.90 mgGALAE/g (in Broccolo Fiolaro). The significant tyrosinase inhibitory effect was observed in the soxhlet extract of Cavolo Nero with 11.93 mgKAE/g. In addition, we evaluated the antioxidant activity of Broccolo Fiolaro and Cavolo Nero on lipopolysaccharide (LPS)-stimulated bladder, kidney and liver specimens, ex vivo. We observed a significant reduction of both nitrite and malondialdehyde (MDA) following treatment that indicates a significant inhibitory effect on oxidative/nitrosative stress and lipoperoxidation, respectively. Additionally, the blunting effect induced by extracts on LPS-induced lactate dehydrogenase (LDH) activity further support a protective effect by both Broccolo Fiolaro and Cavolo Nero in bladder, kidney and liver. HPLC analysis revealed that catechin, epicatechin, vanillic and 3-hydroxy benzoic acids were the major components. The phenolic components may contribute to the observed enzyme inhibitory effects. in vivo tests also demonstrated that the extracts decreased the biochemical parameters in diabetic rats. Particularly, we observed the reduction of plasma glucose levels, urea and total cholesterol following oral administration, with the higher inhibitory effects exerted by Broccolo Fiolaro compared to Cavolo Nero. Overall, our results could provide new insights on the use of these Broccoli species not only as foods but also as functional and nutraceutical supplements.
Atıf Yapan Makale Bilgileri
Kurumlar (7)
Alzaiem Alazhari University
Khartoum, Sudan
Khartoum University
Khartoum, Sudan
National Ribat University
Khartoum, Sudan
Selçuk Üniversitesi
Selçuklu, Turkey
Southeast University
Nanjing, China
University of G. d'Annunzio Chieti and Pescara
Chieti, Italy
University of Nyala
Nyala, Sudan