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A study on in vitro enzyme inhibitory properties of Asphodeline anatolica: New sources of natural inhibitors for public health problems
Scopus
Toplam 133 atıf DOI
Asphodeline species are traditionally used as food and medicines. The different extracts (acetone, methanol and water) from different parts (stem, root, seed and leaf) of Asphodeline anatolica were screened for inhibitory potentials on cholinesterase, tyrosinase, α-amylase and α-glucosidase. Enzyme inhibitory effects were investigated by using microplate reader. All studied extracts exhibited remarkable inhibitory effects on the tested enzymes. Generally, acetone and methanol extracts have higher potentials than water extracts. Also, the enzyme inhibitory activities of the extracts varied significantly according to the plant parts as well as the solvent used. R-Met (7.42. mgGALAEs/g extract) and St-Ac (10.74. mgGALAEs/g extract) had the highest acetylcholinesterase and butrylcholinesterase inhibitory activity, respectively. R-Met (22.48. mgKAEs/g extract) exhibited the strongest tyrosinase inhibitory effects, while Se-Ac had the most potent activity on both α-amylase (2.53. mmolACAEs/g extract) and α-glucosidase (6.70. mmolACAEs/g extract). The results suggested that the A. anatolica extract may be useful for food and medicinal applications.
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Exploring the industrial importance of a miracle herb Withania somnifera (L.) Dunal: Authentication through chemical profiling, in vitro studies and computational analyses
Scopus
Havuzumuzda 19 atıf almış
Withania somnifera (L.) Dunal has been identified as a miracle herb, thus the present study was carried out to investigate W. somnifera growing in Cholistan Desert for its secondary metabolic profile and various biological activities. The WS-C fraction of methanolic extract (WS-M) of W. somnifera was found rich in phenolics (39.96 ± 0.90 mg GAE/g extract), and it exhibited significant DPPH (30.69 ± 0.78 mg TE/g extract) and ABTS (113.60 ± 2.41 mg TE/g extract) free radical inhibitory activities, cupric (Cu+2) and ferric (Fe+3) reducing potential as 157.51 ± 2.10 and 124.60 ± 0.34 mg TE/g extract. The same fraction also exhibited strong total antioxidant capacity (1.39 ± 0.01 mmol TE/g extract) in phosphomolybdenum assay. Other fractions displayed relatively lower potential. In metal-chelating assay, WS-M, WS-E and WS-B showed nearly equal potential with values of 22.35 ± 0.62, 20.96 ± 0.12 and 22.10 ± 0.75 mg EDTAE/g of the extract, while in AChE, BChE and tyrosinase inhibitory assay, again WS-C fraction was most active (7.50 ± 0.21, 8.01 ± 0.53 mg GALAE/g extract and 36.81 ± 0.20 mg KAE/g extract, respectively). In α-amylase and α-glucosidase inhibitory assay, WS-C showed significant potential with values as 0.74 ± 0.01 and 1.14 ± 0.004 mmol ACAE/g extract, respectively. UHPLC-MS analysis of WS-C fraction leads to identify 100 secondary metabolites of phenolic, withanolide, terpenoid, steroid, lignin, flavonoid and limonoid classes of compounds. This profile makes W. somnifera a fascinating plant. These findings were validated by computational studies, which revealed that selected compounds exhibited high binding free energy and inhibition constants with the enzymes tested. Pearson correlation analysis clearly established that the phenols in the tested extracts were the main players as antioxidants and enzyme inhibitors, which was also substantiated through Principal Component Analysis (PCA). These results suggest that chloroform might be suitable for preparing further applications with W. somnifera extracts, and this plant can be placed in the list of top-selling herbs, and may become source of commercial assets.
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Kurumlar (6)
Selçuk Üniversitesi
Selçuklu, Turkey
The Government Sadiq College Women University Bahawalpur
Bahawalpur, Pakistan
The Islamia University of Bahawalpur
Bahawalpur, Pakistan
University of Education
Lahore, Pakistan
University of Karachi
Karachi, Pakistan
University of Mauritius
Reduit, Mauritius