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A study on in vitro enzyme inhibitory properties of Asphodeline anatolica: New sources of natural inhibitors for public health problems
Scopus
Toplam 133 atıf DOI
Asphodeline species are traditionally used as food and medicines. The different extracts (acetone, methanol and water) from different parts (stem, root, seed and leaf) of Asphodeline anatolica were screened for inhibitory potentials on cholinesterase, tyrosinase, α-amylase and α-glucosidase. Enzyme inhibitory effects were investigated by using microplate reader. All studied extracts exhibited remarkable inhibitory effects on the tested enzymes. Generally, acetone and methanol extracts have higher potentials than water extracts. Also, the enzyme inhibitory activities of the extracts varied significantly according to the plant parts as well as the solvent used. R-Met (7.42. mgGALAEs/g extract) and St-Ac (10.74. mgGALAEs/g extract) had the highest acetylcholinesterase and butrylcholinesterase inhibitory activity, respectively. R-Met (22.48. mgKAEs/g extract) exhibited the strongest tyrosinase inhibitory effects, while Se-Ac had the most potent activity on both α-amylase (2.53. mmolACAEs/g extract) and α-glucosidase (6.70. mmolACAEs/g extract). The results suggested that the A. anatolica extract may be useful for food and medicinal applications.
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Atıf Yapan Yayın
Comparative HPLC profiling, antioxidant, enzyme inhibitory activities, and in silico molecular analysis of the aerial parts of Epimedium pubigerum
Scopus
Havuzumuzda 1 atıf almış
Epimedium species are perennial herbaceous plants widely used in folk medicine. However, studies on extracts of their aerial parts are limited. In this study, comparative high-performance liquid chromatography (HPLC) profiling of the methanolic extract of the aerial parts of Epimedium pubigerum (DC.) C. Morren & Decne was performed, and its antioxidant and enzyme inhibitory effects were evaluated. To support the obtained results, in silico molecular analyses were applied to the main identified compounds. The aerial parts of E. pubigerum were extracted using methanol by the maceration method. As a result of high-performance liquid chromatography (HPLC) analysis, a total of 17 compounds were identified in E. pubigerum extracts. The total phenolic and flavonoid contents were found to be 83.76 mg GAE/g and 30.46 mg RE/g, respectively.The extract also exhibited antioxidant effects in different chemical assays (DPPH: 261.37 mg TE/g; ABTS: 423.44 mg TE/g; CUPRAC: 581.01 mg TE/g; FRAP: 313.99 mg TE/g; Metal Chelating: 11.88 mg EDTAE/g; Phosphomolybdenum: 2.21 mmol TE/g). The E. pubigerum extract exhibited enzyme inhibitory capacity (AChE: 9.69 mg GALAE/g; BChE: 2.65 mg GALAE/g; α-amylase: 0.82 mmol ACAE/g; α-glucosidase: 0.85 mmol ACAE/g; tyrosinase: 68.36 mg KAE/g). Molecular docking analyses revealed that the main compounds identified from E. pubigerum exerted inhibitory effects on the cholinergic system through acetylcholinesterase and butyrylcholinesterase targets and exhibited potential inhibitory activity against carbohydrate metabolism via α-amylase and α-glucosidase enzymes. These findings highlight the pharmacological potential of Epimedium species. E. pubigerum extracts showed antioxidant and enzyme inhibitory effects. These findings support the potential of this plant for further research as a therapeutic agent in drug development.
Atıf Yapan Makale Bilgileri
Kurumlar (4)
Artvin Coruh University
Artvin, Turkey
Necmettin Erbakan Üniversitesi
Meram, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
Usak University
Usak, Turkey