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Effect of clinoptilolite on performance of broiler chickens during experimental aflatoxicosis
Scopus
Toplam 134 atıf DOI
1. The amelioration of aflatoxicosis in broiler chickens was examined by feeding 2 concentrations of natural zeolite (clinoptilolite). Clinoptilolite (CLI), incorporated into the diet at 15 and 25 g/kg, was evaluated for its ability to reduce the deleterious effects of 2.5 mg total aflatoxin (AF; 76.40% AFB1, 16.12% AFB2, 6.01% AFG1 and 1.47% AFG in diet on growing broiler chicks from 1 to 21 d of age. A total of 360 broiler chicks were divided into 6 treatment groups (6 replicates of 10 broilers each): control, AF, CLI (15 g/kg), AF plus CLI (15 g/kg), CLI (25 g/kg), and AF plus CLI (25 g/kg). 2. Compared to controls, the AF treatment had significantly decreased body weight gain from week 1 onwards. The adverse effect of AF on food consumption (8.0%) and food conversion ratio (8.3%) was also shown over the entire 21-d feeding period. 3. The addition of CLI (15 g/kg) to an AF-containing diet significantly reduced the deleterious effects of AF on food consumption and body weight gain. Food conversion ratio was also slightly improved by adding CLI (15 g/kg) to AF-containing diets. Food consumption, body weight gain and food conversion ratio values were rendered numerically intermediate between AF and control groups by the addition of CLI (25 g/kg) to the AF-containing diet. 4. The addition of CLI (both 15 and 25 g/kg) to the AF-free diet did not produce any significant changes compared with the controls, except for decreased total food consumption in the CLI (25 g/kg)-alone group. 5. These results suggest that CLI (15 g/kg) addition effectively diminished the detrimental effects of AF on the values investigated. Also, the lower dietary concentration of CLI (15 g/kg) was more effective than the greater concentration against the adverse effects of AF on the variables investigated in this study. © 2000, British Poultry Science Ltd.
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The effects of aflatoxin B1 on growth hormone regulated gene-1 and interaction between DNA and aflatoxin B1 in broiler chickens during hatching
Scopus
Havuzumuzda 4 atıf almış
Many types of aflatoxin cause problems for both public and animal health. Aflatoxin B1 (AFB1) is the most toxic and commonly encountered fungal toxin that appears in poultry feed and in feeds stored under unsuitable conditions. AFB1 decreases feed quality, egg production and fertility of hatching eggs. Also, AFB1 alters the development of embryos by infecting eggs. We investigated using sequence analysis the changes caused by different concentrations of AFB1 on the promoter sequences of the growth hormone regulated gene-1 (GHRG-1) in chick embryo at 13, 17, 19 and 21 days incubation. DNA isolated from the liver of chick embryos treated with different concentrations of AFB1 was separated using agarose gel electrophoresis to detect apoptosis, and DNA interaction with AFB1 was investigated using plasmids to detect changes in electrophoretic mobility and their effects on DNA. Base changes of the promoter sequences of GHRG-1 in 5 ng/egg, 15 ng/egg and 40 ng/egg doses of AFB1 were increased on day 19 compared to base changes of the same AFB1 doses on day 13. We also found that AFB at different concentrations changed the mobility of DNA by binding to it, and that high doses of AFB1 destroyed DNA. The DNA interaction study using plasmid demonstrated that AFB1 at high doses was bound to plasmid DNA, slowed its mobility and inhibited restriction cuts.
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Kurumlar (2)
Karatay Üniversitesi
Konya, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey